RNA

Part:BBa_K2787030:Design

Designed by: Weiyi Tang   Group: iGEM18_ShanghaiTech   (2018-10-09)


pT181 Attenuator target sequence with improved repressive efficiency


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part features extensive secondary structures that we consider full synthesis to be of most efficiency.

Source

The sequence of pT181 sense target was fully synthesized according to the paper of Alexandra M. Westbrook and Julius B. Lucks - Achieving large dynamic range control of gene expression with a compact RNA transcription–translation regulator.

References

Westbrook, A. M. & Lucks, J. B. Achieving large dynamic range control of gene expression with a compact RNA transcription-translation regulator. Nucleic Acids Res 45, 5614-5624, doi:10.1093/nar/gkx215 (2017).